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During susceptible interactions, not only is glutathione content and distribution changed but there are also changes in the expression of genes encoding specific enzyme changes ( Figure 2 )

Although GSH is rapidly oxidized to GSSG, the activity of e-GST remained unchanged, indicating a strong resistance to oxidants (Figure 3)

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Role of impaired Nrf2 activation in the pathogenesis of oxidative stress and inflammation in chronic tubulo-interstitial nephropathy

The eluted protein was concentrated and further purified by size-exclusion chromatography